The
advent of systems biology necessitates the cloning of nearly entire
sets of protein-encoding open reading frames (ORFs), or ORFeomes,
to allow functional studies of the corresponding proteomes. Here
we describe the generation of a first version of the human ORFeome
using a newly improved Gateway recombinational cloning approach.
Using the Mammalian Gene Collection (MGC) resource as a starting
point, we report the successful cloning of 8,076 human ORFs, representing
at least 7,263 human genes, as mini-pools of PCR-amplified products.
These were assembled into the human ORFeome version 1.1 (hORFeome
v1.1) collection. The hORFeome v1.1 represents a central resource
for the cloning of large sets of human ORFs in various settings
for functional proteomics of many types, and will serve as the foundation
for subsequent improved versions of the human ORFeome.
