MAPPING
THE HUMAN INTERACTOME
The first scientific project, the flagship project of the CCSB,
will be to generate a high quality human cancer interactome map
that together with other functional genomic and proteomic information,
will serve as a backbone for the drawing of a global functional
wiring diagram between both already known and yet to be discovered
human cancer gene products.
Since many gene products mediate their function, and/or are regulated
through protein-protein interactions, we have proposed and demonstrated
that one way to accelerate the discovery of candidate cancer genes
and begin to assign their products to potential functional networks
is to comprehensively map such protein-protein interactions to generated
interactome maps. Because of the three limitations above, we are
taking a multi-model-organism (worm-mouse-human) approach to reach
this long-term goal.
We will develop ORFeome resources and interactome maps in three
phases/ modules. We will initiate the first module of 10,000 genes
in Year 1, beginning with the development of ORFs. We will begin
the second module of genes in Year 2 and will begin the third module
of genes in Year 3 .
We will integrate these efforts and work collaboratively with Dr.
Todd Golub and Dr. William Hahn to integrate all three omic mapping
approaches (interactome, transcriptome and phenome) to provide increasingly
reliable hypotheses. Ultimately, we will seek to provide functional
proteome maps to the cancer research community. Such maps should
facilitate the initiation of global studies, starting from already
known components of well-studied pathways, and subsequently provide
the technologies and reagents needed to test the resulting biological
hypotheses back in the most relevant cancer models. |
| |
